Logo of iaiLink to Publisher's site
Infect Immun. 1988 Dec; 56(12): 3157–3161.
PMCID: PMC259717
PMID: 3141282

Regions of Mycoplasma pneumoniae cytadhesin P1 structural gene exist as multiple copies.

C J Su, A Chavoya, and J B Baseman
Author information Copyright and License information Disclaimer

Abstract

The Mycoplasma pneumoniae cytadhesin P1 structural gene with flanking regions was labeled by nick translation and used as a probe to analyze gene copy number in M. pneumoniae. Multiple bands of genomic DNA were hybridized by the probe. To establish what part of the P1 gene existed as multiple copies, the P1 gene and regions adjacent to the 3' and 5' ends were divided with restriction enzymes into 14 segments ranging in size from 174 to 651 base pairs. These pieces were purified on agarose gels, subcloned into pUC19, purified, labeled by nick translation, and used to probe the entire M. pneumoniae genome. Several regions near the middle and carboxy end of the P1 structural gene hybridized to single copies. The remaining P1 subclones hybridized to multiple bands under stringent hybridization conditions, indicating extensive homology with other parts of the M. pneumoniae genome. The single- versus multiple-copy nature of P1 structural gene domains is discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 3157
3157
icon of scanned page 3158
3158
icon of scanned page 3159
3159
icon of scanned page 3160
3160
icon of scanned page 3161
3161
 

Images in this article

Image
Image
on p.3159
Image
Image
on p.3159
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baseman JB, Cole RM, Krause DC, Leith DK. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. [PMC free article] [PubMed]
  • Bergström S, Robbins K, Koomey JM, Swanson J. Piliation control mechanisms in Neisseria gonorrhoeae. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3890–3894. [PMC free article] [PubMed]
  • Blake MS, Gotschlich EC. Gonococcal membrane proteins: speculation on their role in pathogenesis. Prog Allergy. 1983;33:298–313. [PubMed]
  • Dallo SF, Su CJ, Horton JR, Baseman JB. Identification of P1 gene domain containing epitope(s) mediating Mycoplasma pneumoniae cytoadherence. J Exp Med. 1988 Feb 1;167(2):718–723. [PMC free article] [PubMed]
  • Edwards M, McDade RL, Schoolnik G, Rothbard JB, Gotschlich EC. Antigenic analysis of gonococcal pili using monoclonal antibodies. J Exp Med. 1984 Dec 1;160(6):1782–1791. [PMC free article] [PubMed]
  • Feldner J, Göbel U, Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. [PubMed]
  • Hu PC, Cole RM, Huang YS, Graham JA, Gardner DE, Collier AM, Clyde WA., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. [PubMed]
  • Hu PC, Collier AM, Baseman JB. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med. 1977 May 1;145(5):1328–1343. [PMC free article] [PubMed]
  • Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. [PMC free article] [PubMed]
  • Kahane I, Tucker S, Baseman JB. Detection of Mycoplasma pneumoniae adhesin (P1) in the nonhemadsorbing population of virulent Mycoplasma pneumoniae. Infect Immun. 1985 Aug;49(2):457–458. [PMC free article] [PubMed]
  • Krause DC, Leith DK, Wilson RM, Baseman JB. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun. 1982 Mar;35(3):809–817. [PMC free article] [PubMed]
  • Leith DK, Trevino LB, Tully JG, Senterfit LB, Baseman JB. Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens. J Exp Med. 1983 Feb 1;157(2):502–514. [PMC free article] [PubMed]
  • Majiwa PA, Young JR, Englund PT, Shapiro SZ, Williams RO. Two distinct forms of surface antigen gene rearrangement in Trypanosoma brucei. Nature. 1982 Jun 10;297(5866):514–516. [PubMed]
  • Meier JT, Simon MI, Barbour AG. Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia. Cell. 1985 Jun;41(2):403–409. [PubMed]
  • Powell DA, Hu PC, Wilson M, Collier AM, Baseman JB. Attachment of Mycoplasma pneumoniae to respiratory epithelium. Infect Immun. 1976 Mar;13(3):959–966. [PMC free article] [PubMed]
  • Schoolnik GK, Fernandez R, Tai JY, Rothbard J, Gotschlich EC. Gonococcal pili. Primary structure and receptor binding domain. J Exp Med. 1984 May 1;159(5):1351–1370. [PMC free article] [PubMed]
  • Segal E, Hagblom P, Seifert HS, So M. Antigenic variation of gonococcal pilus involves assembly of separated silent gene segments. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2177–2181. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Stern A, Brown M, Nickel P, Meyer TF. Opacity genes in Neisseria gonorrhoeae: control of phase and antigenic variation. Cell. 1986 Oct 10;47(1):61–71. [PubMed]
  • Su CJ, Tryon VV, Baseman JB. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect Immun. 1987 Dec;55(12):3023–3029. [PMC free article] [PubMed]
  • Swanson J, Robbins K, Barrera O, Koomey JM. Gene conversion variations generate structurally distinct pilin polypeptides in Neisseria gonorrhoeae. J Exp Med. 1987 Apr 1;165(4):1016–1025. [PMC free article] [PubMed]
  • Vu AC, Foy HM, Cartwright FD, Kenny GE. The principal protein antigens of isolates of Mycoplasma pneumoniae as measured by levels of immunoglobulin G in human serum are stable in strains collected over a 10-year period. Infect Immun. 1987 Aug;55(8):1830–1836. [PMC free article] [PubMed]
  • Watson HL, McDaniel LS, Blalock DK, Fallon MT, Cassell GH. Heterogeneity among strains and a high rate of variation within strains of a major surface antigen of Mycoplasma pulmonis. Infect Immun. 1988 May;56(5):1358–1363. [PMC free article] [PubMed]
Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)