Logo of iaiLink to Publisher's site
Infect Immun. 1996 Jul; 64(7): 2595–2601.
PMCID: PMC174115
PMID: 8698484

Biofunctional domains of the Mycoplasma pneumoniae P30 adhesin.

Abstract

The P30 adhesin genes of spontaneous, hemadsorption-negative (HA-) class II Mycoplasma pneumoniae mutants that displayed P30 adhesin-deficient protein profiles were analyzed. One subclass of P30-deficient mutants possessed the entire p3O structural gene without alterations (825 nucleotides, encoding 275 amino acids with a predicted molecular mass of 29,743 Da [S. F. Dallo, A. Chavoya, and J. B. Baseman, Infect. Immun. 58:4163-4165, 1990]). However, the second mutant subclass contained a deletion in p3O resulting in the expression of a 25-kDa peptide (681 nucleotides, encoding 227 amino acids with a calculated molecular mass of 24,823 Da). This P25-truncated peptide lacked 8 of the 13 proline-rich amino acid repeat sequences at the carboxy terminus. Whole-cell radioimmunoprecipitation of M. pneumoniae with antibodies directed against the proline-rich repeat sequences located in the carboxy terminus demonstrated their surface accessibility. In contrast, antibodies generated against N-terminal amino acid sequences upstream of the repeats did not bind to intact mycoplasmas. The amino acid sequence homologies exhibited by the P30 adhesin and eucaryotic structural proteins were corroborated by cross-reactive epitopes shared between the P30 adhesin and fibrinogen, keratin, and myosin. These data reinforce the importance of the P30 protein in cytadherence and virulence and provide a molecular basis for postinfectious autoimmunity associated with M. pneumoniae-mediated pathologies.

Full Text

The Full Text of this article is available as a PDF (616K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Atkins JF, Weiss RB, Gesteland RF. Ribosome gymnastics--degree of difficulty 9.5, style 10.0. Cell. 1990 Aug 10;62(3):413–423. [PubMed]
  • Baseman JB. The cytadhesins of Mycoplasma pneumoniae and M. genitalium. Subcell Biochem. 1993;20:243–259. [PubMed]
  • Baseman JB, Cole RM, Krause DC, Leith DK. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. [PMC free article] [PubMed]
  • Baseman JB, Lange M, Criscimagna NL, Giron JA, Thomas CA. Interplay between mycoplasmas and host target cells. Microb Pathog. 1995 Aug;19(2):105–116. [PubMed]
  • Baseman JB, Morrison-Plummer J, Drouillard D, Puleo-Scheppke B, Tryon VV, Holt SC. Identification of a 32-kilodalton protein of Mycoplasma pneumoniae associated with hemadsorption. Isr J Med Sci. 1987 May;23(5):474–479. [PubMed]
  • Dallo SF, Chavoya A, Baseman JB. Characterization of the gene for a 30-kilodalton adhesion-related protein of Mycoplasma pneumoniae. Infect Immun. 1990 Dec;58(12):4163–4165. [PMC free article] [PubMed]
  • Dallo SF, Chavoya A, Su CJ, Baseman JB. DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect Immun. 1989 Apr;57(4):1059–1065. [PMC free article] [PubMed]
  • Dybvig K. DNA rearrangements and phenotypic switching in prokaryotes. Mol Microbiol. 1993 Nov;10(3):465–471. [PubMed]
  • Feldner J, Göbel U, Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. [PubMed]
  • Girón JA, Lange M, Baseman JB. Adherence, fibronectin binding, and induction of cytoskeleton reorganization in cultured human cells by Mycoplasma penetrans. Infect Immun. 1996 Jan;64(1):197–208. [PMC free article] [PubMed]
  • Hedreyda CT, Krause DC. Identification of a possible cytadherence regulatory locus in Mycoplasma pneumoniae. Infect Immun. 1995 Sep;63(9):3479–3483. [PMC free article] [PubMed]
  • Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. [PMC free article] [PubMed]
  • Hu PC, Cole RM, Huang YS, Graham JA, Gardner DE, Collier AM, Clyde WA., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. [PubMed]
  • Inamine JM, Denny TP, Loechel S, Schaper U, Huang CH, Bott KF, Hu PC. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae. Gene. 1988 Apr 29;64(2):217–229. [PubMed]
  • Inamine JM, Loechel S, Collier AM, Barile MF, Hu PC. Nucleotide sequence of the MgPa (mgp) operon of Mycoplasma genitalium and comparison to the P1 (mpp) operon of Mycoplasma pneumoniae. Gene. 1989 Oct 30;82(2):259–267. [PubMed]
  • Inamine JM, Loechel S, Hu PC. Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae. Gene. 1988 Dec 15;73(1):175–183. [PubMed]
  • Krause DC, Leith DK, Baseman JB. Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae. Infect Immun. 1983 Feb;39(2):830–836. [PMC free article] [PubMed]
  • Krause DC, Leith DK, Wilson RM, Baseman JB. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun. 1982 Mar;35(3):809–817. [PMC free article] [PubMed]
  • Layh-Schmitt G, Herrmann R. Spatial arrangement of gene products of the P1 operon in the membrane of Mycoplasma pneumoniae. Infect Immun. 1994 Mar;62(3):974–979. [PMC free article] [PubMed]
  • Layh-Schmitt G, Hilbert H, Pirkl E. A spontaneous hemadsorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1. J Bacteriol. 1995 Feb;177(3):843–846. [PMC free article] [PubMed]
  • MacArthur MW, Thornton JM. Influence of proline residues on protein conformation. J Mol Biol. 1991 Mar 20;218(2):397–412. [PubMed]
  • Meng KE, Pfister RM. Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. J Bacteriol. 1980 Oct;144(1):390–399. [PMC free article] [PubMed]
  • Messing J, Crea R, Seeburg PH. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. [PMC free article] [PubMed]
  • Mizusawa S, Nishimura S, Seela F. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 1986 Feb 11;14(3):1319–1324. [PMC free article] [PubMed]
  • Morrison-Plummer J, Leith DK, Baseman JB. Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae. Infect Immun. 1986 Aug;53(2):398–403. [PMC free article] [PubMed]
  • Reddy SP, Rasmussen WG, Baseman JB. Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. J Bacteriol. 1995 Oct;177(20):5943–5951. [PMC free article] [PubMed]
  • Reddy SP, Rasmussen WG, Baseman JB. Correlations between Mycoplasma pneumoniae sensitivity to cyclosporin A and cyclophilin-mediated regulation of mycoplasma cytadherence. Microb Pathog. 1996 Mar;20(3):155–169. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Sperker B, Hu P, Herrmann R. Identification of gene products of the P1 operon of Mycoplasma pneumoniae. Mol Microbiol. 1991 Feb;5(2):299–306. [PubMed]
  • Stevens MK, Krause DC. Localization of the Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike Triton shell. J Bacteriol. 1991 Feb;173(3):1041–1050. [PMC free article] [PubMed]
  • Su CJ, Chavoya A, Baseman JB. Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect Immun. 1989 Oct;57(10):3237–3239. [PMC free article] [PubMed]
  • Su CJ, Tryon VV, Baseman JB. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect Immun. 1987 Dec;55(12):3023–3029. [PMC free article] [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Tully JG, Rose DL, Baseman JB, Dallo SF, Lazzell AL, Davis CP. Mycoplasma pneumoniae and Mycoplasma genitalium mixture in synovial fluid isolate. J Clin Microbiol. 1995 Jul;33(7):1851–1855. [PMC free article] [PubMed]
  • Wenzel R, Pirkl E, Herrmann R. Construction of an EcoRI restriction map of Mycoplasma pneumoniae and localization of selected genes. J Bacteriol. 1992 Nov;174(22):7289–7296. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)